Differential Dynamic Microscopy
Team members:
Gil Martinez
Dylan Gage
Devynn Wulstein
Alumni
Michelle Wang
Alumni
Christelle Matsuda
Alumni
Hannah Rasmussen
Alumni
Karthik Reddy Peddireddy
Ruilin You
Alumni
Tommy Sottosanti
Alumni
Jonathan Garamella
Gregor Leech
Steven Dang
Alumni
Juexin Marfai
Mehdi Aporvari
Alumni
Justin (JJ) Siu
Alumni
Philip Neill
Alumni
Emmie Kao
Alumni
Juan Carlos Avila
Alumni
Zayda Kellogg
Publications on topic:
 | Topological DNA blends exhibit resonant deformation fields and strain propagation dynamics tuned by steric constraints *Karthik R. Peddireddy*, *Ryan McGorty*, Rae M. Robertson-Anderson Published in Acta Biomaterialia, December 2024 (see publication) Research themes: Differential Dynamic Microscopy |
 | Mapping deformation dynamics to composition of topologically-active DNA blends *Karthik R. Peddireddy*, *Ryan McGorty*, Rae M. Robertson-Anderson Published in Soft Matter, October 2024 (see publication) Research themes: Differential Dynamic Microscopy |
 | Protocol for analyzing DNA dynamics in the presence of crowders and confined within cell-sized droplets *Mehdi Aporvari*, *Ryan McGorty*, Rae M. Robertson-Anderson Published in STAR Protocols, September 2024 (see publication) Research themes: Differential Dynamic Microscopy |
| | Convolutional neural networks applied to differential dynamic microscopy reduces noise when quantifying heterogeneous dynamics *Gildardo Martinez*, *Justin Siu*, *Steven Dang*, *Dylan Gage*, *Emma Kao*, *Juan Carlos Avila*, *Ruilin You*, *Ryan McGorty* Published in Soft Matter, September 2024 (see publication) Research themes: Colloidal Fluids, Differential Dynamic Microscopy |
| | Enzymatic cleaving of entangled DNA rings drives scale-dependent rheological trajectories *Philip Neill*, Natalie Crist, *Ryan McGorty*, Rae Robertson-Anderson Published in Soft Matter, February 2024 (see publication) Research themes: Differential Dynamic Microscopy |
 | Kinesin and myosin motors compete to drive rich multi-phase dynamics in programmable cytoskeletal composites *Ryan McGorty*, Christopher Currie, Jonathan Michel, Mehrzad Sasanpour, Christopher Gunter, K Alice Lindsay, Michael J Rust, Moumita Das, Jennifer L Ross, Rae M. Robertson-Anderson Published in PNAS Nexus, July 2023 (see publication) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy |
 | Motor-driven advection competes with crowding to drive spatiotemporally heterogeneous transport in cytoskeleton composites Janet Y. Sheung, *Jonathan Garamella*, Stella K. Kahl, Brian Y. Lee, *Ryan McGorty*, Rae M. Robertson-Anderson Published in Frontiers in Physics, November 2022 (see publication) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy |
 | Crowding and confinement act in concert to slow DNA diffusion within cell-sized droplets *Mehdi Shafiei Aporvari*, *Steven Dang*, *Juexin Marfai*, Kara Coursey, *Ryan McGorty*, Rae M. Robertson-Anderson Published in iScience, September 2022 (see publication) Research themes: Differential Dynamic Microscopy |
 | Optical-Tweezers-integrating-Differential-Dynamic-Microscopy maps the spatiotemporal propagation of nonlinear strains in polymer blends and composites *Karthik R. Peddireddy*, Ryan Clairmont, *Philip Neill*, *Ryan McGorty*, Rae M. Robertson-Anderson Published in Nature Communications, September 2022 (see publication) Research themes: Differential Dynamic Microscopy |
 | Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy Hannah N. Verwei, Gloria Lee, *Gregor Leech*, Irene Isturiz Petitjean, Gijsje H. Koenderink, Rae M. Robertson-Anderson, *Ryan McGorty* Published in JoVE, June 2022 (see publication) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy |
| | Subtle changes in crosslinking drive diverse anomalous transport characteristics in actin-microtubule networks Sylas Anderson, *Jonathan Garamella*, Serenity Adalbert, *Ryan McGorty*, Rae M Robertson-Anderson Published in Soft Matter, March 2021 (see publication) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy |
 | Myosin-driven actin-microtubule networks exhibit self-organized contractile dynamics Gloria Lee, *Gregor Leech*, Michael J. Rust, Moumita Das, *Ryan McGorty*, Jennifer L. Ross, Rae M Robertson-Anderson Published in Science Advances, February 2021 (see publication) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy |
 | Two-color differential dynamic microscopy for capturing fast dynamics *Ruilin You*, *Ryan McGorty* Published in Review of Scientific Instruments, February 2021 (see publication) Research themes: Differential Dynamic Microscopy |
| | Topology-dependent anomalous dynamics of ring and linear DNA are sensitive to cytoskeleton crosslinking *Devynn Wulstein*, Kathryn Regan, *Jonathan Garamella*, *Ryan McGorty*, Rae M Robertson-Anderson Published in Science Advances, December 2019 (see publication) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy, Light Sheet Microscopy |
 | Filament Rigidity Vies with Mesh Size in Determining Anomalous Diffusion in Cytoskeleton Sylas J. Anderson, *Christelle Matsuda*, *Jonathan Garamella*, *Karthik Reddy Peddireddy Rae M Robertson-Anderson*, *Ryan McGorty* Published in Biomacromolecules, November 2019 (see publication) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy, Light Sheet Microscopy |
| | Measuring capillary wave dynamics using differential dynamic microscopy *Jing Wang*, *Ryan McGorty* Published in Soft Matter, October 2019 (see publication) Research themes: Colloidal Fluids, Differential Dynamic Microscopy |
| | Bridging the spatiotemporal scales of macromolecular transport in crowded biomimetic systems Kathryn Regan, *Devynn Wulstein*, *Hannah Rasmussen*, *Ryan McGorty*, Rae M. Robertson-Anderson Published in Soft Matter, February 2019 (see publication) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy, Light Sheet Microscopy |
 | Point-spread function engineering enhances digital Fourier microscopy *Devynn Wulstein*, *Ryan McGorty* Published in Optics Letters, October 2017 (see publication) Research themes: Differential Dynamic Microscopy |
 | Light-sheet microscopy with digital Fourier analysis measures transport properties over large field-of-view *Devynn Wulstein*, Kathryn Regan, Rae M. Robertson-Anderson, *Ryan McGorty* Published in Optics Express, September 2016 (see publication) Research themes: Differential Dynamic Microscopy, Light Sheet Microscopy |
Presentations:
 | Optical tweezers map spatiotemporal force generation in active actin-microtubule composites *Anindya Chowdhury*, *Karthik Reddy Peddireddy*, Moumita Das, Mehrzad Sasanpour, Jennifer Ross, *Ryan McGorty*, Megan T. Valentine, Michael J. Rust, Rae M. Robertson-Anderson Presented at APS March Meeting, March 2024 (see published abstract) Research themes: Differential Dynamic Microscopy |
| | Improved quantification of colloidal dynamics with machine learning and simulations *Dylan Gage*, *Gildardo Martinez*, *Justin Siu*, *Emma Kao*, *Juan Carlos Avila*, *Ruilin You*, *Ryan McGorty* Presented at APS March Meeting, March 2024 (see published abstract) Research themes: Differential Dynamic Microscopy |
| | Dynamics and rheology of active biomaterials and gels quantified with optical microscopy tools *Ryan McGorty* Presented at APS March Meeting, March 2024 (see published abstract) Research themes: Differential Dynamic Microscopy |
| | Dynamics and rheology of colloidal rod suspensions measured with differential dynamic microscopy *Laura G Morocho*, *Nikhil Sonthalia*, *Ryle R. Rel*, Esther Yang, *Ryan McGorty* Presented at APS March Meeting, March 2024 (see published abstract) Research themes: Differential Dynamic Microscopy, Rheology |
| | Quantifying dynamics of soft and active matter with microscopy and machine learning *Gildardo Martinez*, *Justin Siu*, *Dylan Gage*, *Emma Kao*, *Juan Carlos Avila*, *Ruilin You*, *Ryan McGorty* Presented at APS March Meeting, March 2024 (see published abstract) Research themes: Differential Dynamic Microscopy |
 | Nonlinear local straining leads to non-equilibrium deformation fields and dynamics of motor-driven cytoskeleton composites Mehrzad Sasanpour, Daisy H. Achiriloaie, Maya Hendija, *Karthik R. Peddireddy*, *Ryan McGorty*, Rae Robertson-Anderson Presented at APS March Meeting, March 2023 (see published abstract) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy |
| | Differential dynamic microscopy enhanced with convolutional neural networks *Justin Siu*, *Ruilin You*, *Steven Dang*, *Ryan McGorty* Presented at APS March Meeting, March 2023 (see published abstract) Research themes: Differential Dynamic Microscopy |
| | Nonlinear Mechanics and Stress Propagation in Out-of-Equilibrium Cytoskeleton Composites Daisy H. Achiriloaie, Kit K. Bennett, Mehrzad Sasanpour, *Karthik Peddireddy*, Michael J. Rust, Moumita Das, Megan T. Valentine, Janet Y. Sheung, Jennifer L. Ross, *Ryan McGorty*, Rae Robertson-Anderson Presented at APS March Meeting, March 2023 (see published abstract) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy |
| | Motor-driven advection competes with crowding to drive spatiotemporally heterogeneous transport in cytoskeleton composites Brian Y. Lee, Janet Y. Sheung, *Jonathan Garamella*, Stella Kahl, *Ryan McGorty*, Rae Robertson-Anderson Presented at APS March Meeting, March 2023 (see published abstract) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy |
| | Motor-driven advection competes with macromolecular crowding to drive spatiotemporally heterogeneous transport in cytoskeleton composites Janet Y. Sheung, *Jonathan Garamella*, Stella Kahl, Brian Y. Lee, Nadia Schwartz Bolef, Daisy H. Achiriloaie, Jennifer L. Ross, *Ryan McGorty*, Rae Robertson-Anderson Presented at APS March Meeting, March 2023 (see published abstract) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy |
| | DNA transport within confined motor-driven cytoskeletal networks *Mehdi Shafiei Aporvari*, *Philip Neill*, Daisy H. Achiriloaie, *Ryan McGorty*, Rae Robertson-Anderson Presented at APS March Meeting, March 2023 (see published abstract) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy |
| | Time-varying stress response and deformation dynamics of topologically-active DNA solutions *Karthik Peddireddy*, *Philip Neill*, *Juexin Marfai*, *Ryan McGorty*, Rae Robertson-Anderson Presented at APS March Meeting, March 2023 (see published abstract) Research themes: Differential Dynamic Microscopy |
 | Mapping nonlinear stress propagation in non-equilibrium complex fluids *Karthik Peddireddy*, Ryan Clairmont, *Philip Neill*, *Jonathan Garamella*, *Ryan McGorty*, Rae M. Robertson-Anderson Presented at APS March Meeting, March 2022 (see published abstract) Research themes: Differential Dynamic Microscopy |
| | DNA dynamics in crowded cell-sized droplets *Mehdi Shafiei Aporvari*, *Steven Dang*, *Juexin Marfai*, Kara Coursey, *Ryan McGorty*, Rae M. Robertson-Anderson Presented at APS March Meeting, March 2022 (see published abstract) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy |
| | Effects of crowding and confinement on the transport and localization of DNA *Steven Dang*, *Mehdi Shafiei Aporvari*, *Juexin Marfai*, Kara Coursey, *Ryan McGorty*, Rae M. Robertson-Anderson Presented at APS March Meeting, March 2022 (see published abstract) Research themes: Differential Dynamic Microscopy |
| | Tunable Non-Equilibrium Dynamics of Motor-Driven Cytoskeleton Composites Daisy H. Achiriloaie, Christopher Currie, Maya Hendija, *Ryan McGorty*, Jennifer L. Ross, Moumita Das, Michael J. Rust, Janet Y. Sheung, Rae M. Robertson-Anderson Presented at APS March Meeting, March 2022 (see published abstract) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy |
 | DNA transport and conformational dynamics in active cytoskeleton composites *Jonathan Garamella*, Serenity Adalbert, *Gina Aguirre*, *Ryan McGorty*, Rae M. Robertson-Anderson Presented at APS March Meeting, March 2021 (see published abstract) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy |
| | Mapping the nonlinear stress propagation in topological polymer blends *Karthik Peddireddy*, *Gina Aguirre*, *Jonathan Garamella*, *Ryan McGorty*, Rae M. Robertson-Anderson Presented at APS March Meeting, March 2021 (see published abstract) Research themes: Differential Dynamic Microscopy |
| | Differential Dynamic Microscopy of Active Actin-Microtubule Networks *Gregor Leech*, Gloria Lee, Michael Rust, Moumita Das, Jennifer L. Ross, *Ryan McGorty*, Rae M. Robertson-Anderson Presented at APS March Meeting, March 2021 (see published abstract) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy |
| | Tuning Dynamics of Myosin-Driven Actin-Microtubule Networks Gloria Lee, *Gregor Leech*, Christopher Currie, Michael Rust, Jennifer L. Ross, *Ryan McGorty*, Rae M. Robertson-Anderson Presented at APS March Meeting, March 2021 (see published abstract) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy |
 | Anomalous transport across scales in crosslinked actin-microtubule composites Sylas Anderson, *Jonathan Garamella*, *Ryan McGorty*, Rae M. Robertson-Anderson Presented at APS March Meeting, March 2020 (see published abstract) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy |
| | Non-ergodic transport and conformational dynamics of DNA in biomimetic cytoskeleton networks *Jonathan Garamella*, *Gina Aguirre*, Kathryn Regan, *Ryan McGorty*, Rae M. Robertson-Anderson Presented at APS March Meeting, March 2020 (see published abstract) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy |
| | Temporal super-resolution differential dynamics microscopy for detecting fast dynamics *Ruilin You*, *Ryan McGorty* Presented at APS March Meeting, March 2020 (see published abstract) Research themes: Differential Dynamic Microscopy |
| | Observing phase separation in colloid-polymer mixtures with a custom light-sheet rheoscope *Jing Wang*, *Ryan McGorty* Presented at APS March Meeting, March 2020 (see published abstract) Research themes: Colloidal Fluids, Differential Dynamic Microscopy |
| | Connecting microscale stresses to macromolecular motion in entangled ring-linear DNA blends *Karthik Peddireddy*, Megan C. Lee, *Jonathan Garamella*, *Ryan McGorty*, Rae M. Robertson-Anderson Presented at APS March Meeting, March 2020 (see published abstract) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy |
| Non-Ergodic Transport and Conformational Dynamics of DNA in Biomimetic Cytoskeleton Networks *Jonathan Garamella*, *Gina Aguirre*, *Ryan McGorty*, Rae M. Robertson-Anderson Presented at Biophysical Society Annual Meeting, February 2020 (see published abstract) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy |
 | Ensemble dynamics of large DNA molecules within entangled and crosslinked cytoskeleton networks *Devynn Wulstein*, Kathryn Regan, Shea Ricketts, Rae M. Robertson-Anderson, *Ryan McGorty* Presented at APS March Meeting, March 2019 (see published abstract) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy |
| | Measuring the dispersion relation of capillary waves using differential dynamic microscopy *Jing Wang*, *Ryan McGorty* Presented at APS March Meeting, March 2019 (see published abstract) Research themes: Colloidal Fluids, Differential Dynamic Microscopy |
| | Measure particle diffusion in cytoskeletal networks with light-sheet and differential dynamic microscopy *Christelle Matsuda*, Sylas Anderson, Rae M. Robertson-Anderson, *Ryan McGorty* Presented at APS March Meeting, March 2019 (see published abstract) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy |
| | Diffusion and Conformational Dynamics of Linear and Circular DNA in Crosslinked Cytoskeleton Composites Kathryn Regan, *Devynn Wulstein*, Shea Ricketts, *Ryan McGorty*, Rae M. Robertson-Anderson Presented at APS March Meeting, March 2019 (see published abstract) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy |
 | Selective-plane illumination microscopy to characterize diffusion of DNA in cytoskeletal networks *Devynn Wulstein*, Kathryn Regan, Shea Ricketts, Rae M. Robertson-Anderson, *Ryan McGorty* Presented at APS March Meeting, March 2018 (see published abstract) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy, Light Sheet Microscopy |