Publications

2024

Topological DNA blends exhibit resonant deformation fields and strain propagation dynamics tuned by steric constraints *Karthik R. Peddireddy*, *Ryan McGorty*, Rae M. Robertson-Anderson Published in Acta Biomaterialia, December 2024 (see publication) Research themes: Differential Dynamic Microscopy

Mapping deformation dynamics to composition of topologically-active DNA blends *Karthik R. Peddireddy*, *Ryan McGorty*, Rae M. Robertson-Anderson Published in Soft Matter, October 2024 (see publication) Research themes: Differential Dynamic Microscopy

Protocol for analyzing DNA dynamics in the presence of crowders and confined within cell-sized droplets *Mehdi Aporvari*, *Ryan McGorty*, Rae M. Robertson-Anderson Published in STAR Protocols, September 2024 (see publication) Research themes: Differential Dynamic Microscopy

Convolutional neural networks applied to differential dynamic microscopy reduces noise when quantifying heterogeneous dynamics *Gildardo Martinez*, *Justin Siu*, *Steven Dang*, *Dylan Gage*, *Emma Kao*, *Juan Carlos Avila*, *Ruilin You*, *Ryan McGorty* Published in Soft Matter, September 2024 (see publication) Research themes: Colloidal Fluids, Differential Dynamic Microscopy

Enzymatic cleaving of entangled DNA rings drives scale-dependent rheological trajectories *Philip Neill*, Natalie Crist, *Ryan McGorty*, Rae Robertson-Anderson Published in Soft Matter, February 2024 (see publication) Research themes: Differential Dynamic Microscopy

Design and Building of a Customizable, Single-Objective, Light-Sheet Fluorescence Microscope for the Visualization of Cytoskeleton Networks Nathan Felcher, Daisy Achiriloaie, Brian Lee, *Ryan McGorty*, Janet Sheung Published in JoVE, January 2024 (see publication) Research themes: Cytoskeletal Networks, Light Sheet Microscopy

2023

Kinesin and myosin motors compete to drive rich multi-phase dynamics in programmable cytoskeletal composites *Ryan McGorty*, Christopher Currie, Jonathan Michel, Mehrzad Sasanpour, Christopher Gunter, K Alice Lindsay, Michael J Rust, Moumita Das, Jennifer L Ross, Rae M. Robertson-Anderson Published in PNAS Nexus, July 2023 (see publication) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy

Cooperative Rheological State-Switching of Enzymatically-Driven Composites of Circular Dna And Dextran *Juexin Marfai*, *Ryan McGorty*, Rae M. Robertson-Anderson Published in Advanced Materials, July 2023 (see publication) Research themes: Rheology

2022

Motor-driven advection competes with crowding to drive spatiotemporally heterogeneous transport in cytoskeleton composites Janet Y. Sheung, *Jonathan Garamella*, Stella K. Kahl, Brian Y. Lee, *Ryan McGorty*, Rae M. Robertson-Anderson Published in Frontiers in Physics, November 2022 (see publication) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy

Crowding and confinement act in concert to slow DNA diffusion within cell-sized droplets *Mehdi Shafiei Aporvari*, *Steven Dang*, *Juexin Marfai*, Kara Coursey, *Ryan McGorty*, Rae M. Robertson-Anderson Published in iScience, September 2022 (see publication) Research themes: Differential Dynamic Microscopy

Optical-Tweezers-integrating-Differential-Dynamic-Microscopy maps the spatiotemporal propagation of nonlinear strains in polymer blends and composites *Karthik R. Peddireddy*, Ryan Clairmont, *Philip Neill*, *Ryan McGorty*, Rae M. Robertson-Anderson Published in Nature Communications, September 2022 (see publication) Research themes: Differential Dynamic Microscopy

Reconstituting and Characterizing Actin-Microtubule Composites with Tunable Motor-Driven Dynamics and Mechanics Mehrzad Sasanpour, Daisy H. Achiriloaie, Gloria Lee, *Gregor Leech*, Christopher Currie, K. Alice Lindsay, Jennifer L. Ross, *Ryan McGorty*, Rae M. Robertson-Anderson Published in JoVE, August 2022 (see publication) Research themes: Cytoskeletal Networks

Shear-induced vorticity aligned flocs in a temperature responsive colloid-polymer mixture *Ryle Rel*, *Dennis Terwilliger*, *Ryan McGorty* Published in Frontiers in Physics, August 2022 (see publication) Research themes: Colloidal Fluids, Rheology

Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy Hannah N. Verwei, Gloria Lee, *Gregor Leech*, Irene Isturiz Petitjean, Gijsje H. Koenderink, Rae M. Robertson-Anderson, *Ryan McGorty* Published in JoVE, June 2022 (see publication) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy

DNA topology dictates emergent bulk elasticity and hindered macromolecular diffusion in DNA-dextran composites Pawan Khanal, *Karthik R. Peddireddy*, *Juexin Marfai*, *Ryan McGorty*, Rae M. Robertson-Anderson Published in Journal of Rheology, June 2022 (see publication) Research themes: Rheology

2021

Active cytoskeletal composites display emergent tunable contractility and restructuring Gloria Lee, *Gregor Leech*, Pancy Lwin, Jonathan Michel, Christopher Currie, Michael J. Rust, Jennifer L. Ross, *Ryan McGorty*, Moumita Das, Rae M. Robertson-Anderson Published in Soft Matter, October 2021 (see publication) Research themes: Cytoskeletal Networks

Light sheet fluorescence microscopy illuminating soft matter *Ruilin You*, *Ryan McGorty* Published in Frontiers in Physics, September 2021 (see publication) Research themes: Colloidal Fluids, Light Sheet Microscopy

Core–shell droplets and microcapsules formed through liquid–liquid phase separation of a colloid–polymer mixture *Steven Dang*, *John Brady*, *Ryle Rel*, *Sreenidhi Surineni*, *Conor O'Shaughnessy*, *Ryan McGorty* Published in Soft Matter, August 2021 (see publication) Research themes: Colloidal Fluids

Subtle changes in crosslinking drive diverse anomalous transport characteristics in actin-microtubule networks Sylas Anderson, *Jonathan Garamella*, Serenity Adalbert, *Ryan McGorty*, Rae M Robertson-Anderson Published in Soft Matter, March 2021 (see publication) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy

Myosin-driven actin-microtubule networks exhibit self-organized contractile dynamics Gloria Lee, *Gregor Leech*, Michael J. Rust, Moumita Das, *Ryan McGorty*, Jennifer L. Ross, Rae M Robertson-Anderson Published in Science Advances, February 2021 (see publication) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy

Two-color differential dynamic microscopy for capturing fast dynamics *Ruilin You*, *Ryan McGorty* Published in Review of Scientific Instruments, February 2021 (see publication) Research themes: Differential Dynamic Microscopy

2020

Anomalous and heterogeneous DNA transport in biomimetic cytoskeleton networks *Jonathan Garamella*, Kathryn Regan, *Gina Aguirre*, *Ryan J McGorty*, Rae M Robertson-Anderson Published in Soft Matter, July 2020 (see publication) Research themes: Cytoskeletal Networks, Light Sheet Microscopy

Liquid-Liquid Phase Separation: Undergraduate Labs on a New Paradigm for Intracellular Organization *Caroline P. Riedstra*, *Ryan McGorty* Published in The Biophysicist, February 2020 (see publication) Research themes:

2019

Topology-dependent anomalous dynamics of ring and linear DNA are sensitive to cytoskeleton crosslinking *Devynn Wulstein*, Kathryn Regan, *Jonathan Garamella*, *Ryan McGorty*, Rae M Robertson-Anderson Published in Science Advances, December 2019 (see publication) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy, Light Sheet Microscopy

Filament Rigidity Vies with Mesh Size in Determining Anomalous Diffusion in Cytoskeleton Sylas J. Anderson, *Christelle Matsuda*, *Jonathan Garamella*, *Karthik Reddy Peddireddy Rae M Robertson-Anderson*, *Ryan McGorty* Published in Biomacromolecules, November 2019 (see publication) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy, Light Sheet Microscopy

Before the breach: Interactions between colloidal particles and liquid interfaces at nanoscale separations Anna Wang, Jos W. Zwanikken, David M. Kaz, *Ryan McGorty*, Aaron M. Goldfain, W. Benjamin Rogers, Vinothan N. Manoharan Published in Physical Review E, October 2019 (see publication) Research themes: Colloidal Fluids, Holographic Microscopy

Measuring capillary wave dynamics using differential dynamic microscopy *Jing Wang*, *Ryan McGorty* Published in Soft Matter, October 2019 (see publication) Research themes: Colloidal Fluids, Differential Dynamic Microscopy

Bridging the spatiotemporal scales of macromolecular transport in crowded biomimetic systems Kathryn Regan, *Devynn Wulstein*, *Hannah Rasmussen*, *Ryan McGorty*, Rae M. Robertson-Anderson Published in Soft Matter, February 2019 (see publication) Research themes: Cytoskeletal Networks, Differential Dynamic Microscopy, Light Sheet Microscopy

2017

Point-spread function engineering enhances digital Fourier microscopy *Devynn Wulstein*, *Ryan McGorty* Published in Optics Letters, October 2017 (see publication) Research themes: Differential Dynamic Microscopy

Super-resolution microscopy reveals that disruption of ciliary transition-zone architecture causes Joubert syndrome Xiaoyu Shi, Galo Garcia III, Julie C. Van De Weghe, *Ryan McGorty*, Gregory J. Pazour, Dan Doherty, Bo Huang, Jeremy F. Reiter Published in Nature Cell Biology, August 2017 (see publication) Research themes:

High-NA open-top selective-plane illumination microscopy for biological imaging *Ryan McGorty*, Dan Xie, Bo Huang Published in Optics Express, July 2017 (see publication) Research themes: Light Sheet Microscopy

2016

Contact-line pinning controls how quickly colloidal particles equilibrate with liquid interfaces Anna Wang, *Ryan McGorty*, David M. Kaz, Vinothan N. Manoharan Published in Soft Matter, November 2016 (see publication) Research themes: Colloidal Fluids, Holographic Microscopy

Light-sheet microscopy with digital Fourier analysis measures transport properties over large field-of-view *Devynn Wulstein*, Kathryn Regan, Rae M. Robertson-Anderson, *Ryan McGorty* Published in Optics Express, September 2016 (see publication) Research themes: Differential Dynamic Microscopy, Light Sheet Microscopy

Force Feedback Controls Motor Activity and Mechanical Properties of Self-Assembling Branched Actin Networks Peter Bieling, Tai-De Li, Julian Weichsel, *Ryan McGorty*, Pamela Jreij, Bo Huang, Daniel A. Fletcher, R. Dyche Mullins Published in Cell, January 2016 (see publication) Research themes: